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Characterization of CD4 + T cell subsets and HIV susceptibility in the inner and outer foreskin of Ugandan men
Author(s) -
Galiwango Ronald Moses,
Yegorov Sergey,
Joag Vineet,
Prodger Jessica,
Shahabi Kamnoosh,
Huibner Sanja,
Muyanja Enoch,
Kabuubi Brian Roy,
Namuniina Annmarie,
Nalutaaya Annet,
Ssemaganda Aloysius,
Lutwama Fredrick,
Kitandwe Paul Kato,
Nanvubya Annet,
Mpendo Juliet,
Bagaya Bernard,
Kiwanuka Noah,
Kaul Rupert
Publication year - 2019
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.13143
Subject(s) - foreskin , proinflammatory cytokine , immunology , immune system , cytokine , t cell , biology , microbiology and biotechnology , medicine , inflammation , cell culture , genetics
Problem Biological mechanisms of foreskin HIV acquisition are poorly defined. The inner foreskin is preferentially infected in explant models, so we hypothesized that this site would be enriched for HIV‐susceptible CD4 + T cells and proinflammatory/chemoattractant cytokines. Method of study A total of 42 HIV‐uninfected Ugandan men without genital symptoms provided foreskin tissues and swabs at the time of elective penile circumcision. The immune phenotype of foreskin‐derived CD4 + T cells and entry of a CCR5‐tropic HIV pseudovirus was characterized, and specific cytokine levels assayed by multiplexed chemiluminescent ELISA. Results Unexpectedly, outer foreskin CD4 + T cells more frequently expressed CCR5 (median 29.2% vs 22.9%, P = 0.01) and CD69 (median 36.5% vs 15%, P < 0.01), and on a per‐cell basis, HIV entry was higher. However, overall CD4 + T cell density was approximately twofold higher in the inner foreskin, and several highly susceptible T cell subsets were increased at this site, including Th17 cells (20.0% vs 14.1%, P = 0.0021). Specific pro‐inflammatory cytokine levels were also higher on the inner foreskin surface (IL‐17, IL‐8, RANTES and IL‐1β; all P < 0.05). Conclusion There was marked heterogeneity in CD4 + T cell populations and immune milieu between inner and outer foreskin tissues. Despite higher per‐cell viral entry into CD4 + T cells from the outer foreskin, the higher target cell density and enriched pro‐inflammatory cytokines of the inner foreskin suggest that this may be a preferential site for HIV acquisition.