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Day‐7 embryos generate an anti‐inflammatory immune response in peripheral blood immune cells in superovulated cows
Author(s) -
Talukder Anup K.,
Rashid Mohammad B.,
Takedomi Toshiro,
Moriyasu Satoru,
Imakawa Kazuhiko,
Miyamoto Akio
Publication year - 2019
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.13069
Subject(s) - immune system , peripheral blood mononuclear cell , uterus , andrology , embryo , biology , insemination , immunology , endocrinology , medicine , in vitro , sperm , biochemistry , microbiology and biotechnology
Problem Recently, we demonstrated both in vitro and in vivo that an immunological crosstalk between Day‐7 embryo and immune cells exists locally in the uterus in cows. The peripheral immune response to early embryos at Day‐7 of pregnancy in cows remains largely unknown. Thus, we aimed to investigate the response of peripheral blood immune cells in the presence of multiple Day‐7 embryos in the uterus in donor cows of embryo transfer program. Method of study Superovulated cows were either inseminated (n = 13) at 12‐18 hours post‐estrus or remained without inseminations (n = 6). Blood was collected following insemination (Day‐1) and immediately after uterine flushing (Day‐7). Polymorphonuclear neutrophils (PMNs) and peripheral blood mononuclear cells (PBMCs) were isolated from whole blood for analysis of gene expression. Results Interferon‐stimulated genes ( ISGs: ISG15 and OAS1 ) were increased in both PMNs and PBMCs, with up‐regulation of PTGES and anti‐inflammatory cytokines ( TGFB1 and IL10 ) expressions at Day‐7 of post‐inseminations in cows, when compared to those at Day‐7 in non‐inseminated cows. Conclusion The findings indicate that the presence of multiple embryos in the uterus generates an anti‐inflammatory immune response in peripheral blood immune cells at Day‐7 of pregnancy in cows, which might help to accept semi‐allogenic embryo in the uterus.