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Genome‐wide identification of micro RNA s in decidual natural killer cells from patients with unexplained recurrent spontaneous abortion
Author(s) -
Li Dandan,
Li Jian,
Jia Bin,
Wang Yue,
Zhang Juxin,
Liu Guangzhi
Publication year - 2018
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.13052
Subject(s) - rna , gene , biology , microarray , gene expression , decidua , microbiology and biotechnology , andrology , genetics , pregnancy , medicine , fetus , placenta
Problem This study revealed mi RNA regulation and functional microarray‐based profiles of mi RNA s in the natural killer ( NK ) cells of the decidual tissue obtained from patients with unexplained recurrent spontaneous abortion ( URSA ). Method of Study Patients with URSA were categorized based on the occurrence of at least two or more successive spontaneous abortions between 7th and 10th gestational week. Total RNA was isolated from the NK cells of the decidual tissue obtained from patients with induced abortion at about the 8th gestational week. The potential contribution of regulatory mi RNA s to a genetic predisposition to URSA was characterized by comparison with healthy and fertile controls and bioinformatics analyses. Results Analysis of the mi RNA expression profiles identified 50 mi RNA s that were differentially expressed, including one down‐regulated and 49 up‐regulated mi RNA s in the URSA group. Mi RNA ‐Gene‐Network, mi RNA ‐ GO ‐Network and mi RNA ‐Gene‐ TF ‐Network were constructed. The key mi RNA s, genes, GO s and core TF s in the network were determined. Conclusion Our results suggest that a close relationship exists between the aberrant mi RNA s and URSA . Furthermore, these findings support the notion that altered expression of mi RNA s may contribute to the clinical diagnosis of URSA and the potential to develop novel strategies for therapeutic targets against URSA .