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Progestin‐based contraception regimens modulate expression of putative HIV risk factors in the vaginal epithelium of pig‐tailed Macaques
Author(s) -
Bosinger Steven E.,
Tharp Gregory K.,
Patel Nirav B.,
Zhao Chunxia,
Payne Tamika L.,
Dietz Ostergaard Sharon,
Butler Katherine,
Ellis Sha,
Johnson Ryan L.,
Kersh Ellen N.,
McNicholl Janet M.,
Vishwanathan Sundaram A.
Publication year - 2018
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.13029
Subject(s) - progestin , medroxyprogesterone acetate , follicular phase , levonorgestrel , luteal phase , menstrual cycle , medicine , estrogen , physiology , population , andrology , biology , endocrinology , family planning , hormone , environmental health , research methodology
Problem In women, the use of progestin‐based contraception may increase the risk of vaginal HIV acquisition. We previously showed in macaques that there is a significantly higher simian‐human immunodeficiency virus ( SHIV ) acquisition rate in the luteal phase of the menstrual cycle, which presents a naturally high‐progesterone state, and this may be attributable to altered expression of innate immune factors. We hypothesized that progestin‐based contraception, especially depot medroxyprogesterone acetate ( DMPA ), would, in a similar way, affect mucosal immune factors that influence HIV acquisition risk. Method of study We used a pig‐tailed macaque model to evaluate the effects of two progestin‐based contraceptives, DMPA , and levonorgestrel ( LNG )/ethinyl estradiol ( EE )‐based combined oral contraceptives ( COC s), on innate mucosal factors. We compared the vaginal epithelial thickness data from previous studies and used cytokine profiling and microarray analysis to evaluate contraception‐induced molecular changes in the vagina. Results The administration of DMPA caused a reduction in the thickness of the vaginal epithelium relative to that of the follicular or luteal phase. DMPA also induced a significant increase in vaginal levels of the anti‐inflammatory cytokine IL ‐10. Both DMPA ‐ and LNG ‐based contraception induced a signature of gene expression similar to that of the luteal phase, only more exacerbated, including widespread downregulation of antiviral genes. Conclusion The use of progestin‐based contraception might engender a milieu that poses an increased risk of HIV acquisition as compared to both the luteal and follicular phases of the menstrual cycle.