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Downregulation of ILT 4 + dendritic cells in recurrent miscarriage and recurrent implantation failure
Author(s) -
Liu Su,
Wei Hongxia,
Li Yuye,
Huang Chunyu,
Lian Ruochun,
Xu Jian,
Chen Lanna,
Zeng Yong
Publication year - 2018
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12998
Subject(s) - recurrent miscarriage , foxp3 , endometrium , immunohistochemistry , flow cytometry , immune system , downregulation and upregulation , andrology , immune tolerance , implantation failure , peripheral blood mononuclear cell , medicine , immunology , biology , pregnancy , miscarriage , in vitro , infertility , gene , biochemistry , genetics
Problem The role of ILT 4 + DC s in healthy fertile controls and patients with recurrent miscarriages ( RM ) and recurrent implantation failure ( RIF ) is unclear. Method of study We studied the expression of ILT 4 from peripheral blood and endometrial samples from healthy controls and patients with RM and RIF by flow cytometry and immunohistochemistry analysis. Endometrial Foxp3 expression was also investigated using immunohistochemistry. Results In peripheral blood, there was a significant increase in the percentage of ILT 4 + DC s in healthy fertile controls compared with patients with RM and RIF . The presence of ILT 4 + DC is even more prominent in the endometrium of healthy fertile controls compared with patients with RM and RIF . Moreover, there was a strong correlation between the number of ILT 4 + cells and Foxp3 + Tregs in healthy fertile controls, but not in patients with RM and RIF . Conclusion Our data indicate that ILT 4 + DC s play an important role in the maintenance of immune tolerance during pregnancy, probably through the induction of Foxp3 + Treg cells, a process which is impaired in RM and RIF .