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XCL 1‐ XCR 1 pathway promotes trophoblast invasion at maternal‐fetal interface by inducing MMP ‐2/ MMP ‐9 activity
Author(s) -
Zhang SiMing,
Tian FuJu,
Zeng WeiHong,
Ma XiaoLing,
Ren JiaBin,
Lin Yi
Publication year - 2018
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12990
Subject(s) - trophoblast , decidua , chemokine , microbiology and biotechnology , protein kinase b , chemokine receptor , chemistry , matrix metalloproteinase , placenta , cell migration , receptor , biology , signal transduction , andrology , cell , medicine , biochemistry , fetus , genetics , pregnancy
Problem Certain chemokines with their receptors can promote or inhibit trophoblast cell migration and invasion in human first‐trimester placenta. Whether the lymphotactin (Lptn; XCL 1)— XC chemokine receptor 1 ( XCR 1) chemokine pathway affects trophoblast cell migration and invasion in human first‐trimester placenta remains unclear. Method of study The expression pattern of chemokine XCL 1 and its receptor XCR 1 was detected in human first‐trimester by qRT ‐ PCR , and the effect of recombinant human XCL 1 (rh XCL 1) on trophoblast cell function was tested by wound healing and Transwell assays. Matrix metalloproteinase ( MMP ) activity in trophoblast cells treated with rh XCL 1 was assessed via qRT ‐ PCR and gelatin zymography. Results Abundant XCR 1 mRNA was expressed in the first‐trimester decidua and villi. XCL 1 and XCR 1 mRNA were expressed at a higher level in the first‐trimester than in the term placenta. Rh XCL 1 promoted trophoblast cell migration and invasion by increasing MMP ‐9 and MMP ‐2 activity and that of the MMP ‐2/tissue inhibitor of metalloproteinases 2 ( TIMP ‐2) complex via the phosphatidylinositol 3‐kinase ( PI 3K)/ AKT kinase ( AKT ), mitogen‐activated protein kinase ( MEK ), and JUN N‐terminal kinase ( JNK ) signaling pathways. Conclusion XCL 1‐ XCR 1 chemokine pathway promotes trophoblast invasion by increasing matrix metalloproteinase activity in human first‐trimester placenta.