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Eukaryotic initiation factor 5A and Ca 2+ /calmodulin‐dependent protein kinase 1D modulate trophoblast cell function
Author(s) -
Qin Xiaoli,
Liang Yan,
Guo Yuna,
Liu Xiaorui,
Zeng Weihong,
Wu Fan,
Lin Yi,
Zhang Yan
Publication year - 2018
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12845
Subject(s) - trophoblast , microbiology and biotechnology , biology , transfection , apoptosis , flow cytometry , cell growth , terminal deoxynucleotidyl transferase , protein kinase a , kinase , tunel assay , cell culture , biochemistry , placenta , fetus , pregnancy , genetics
Problem Trophoblast cells regulate embryo implantation and placental development. Eukaryotic initiation factor 5A ( eIF 5A) is an initiator of translation involved in cellular processes, such as migration, proliferation, and apoptosis. However, the function of eIF 5A in trophoblast cells is unknown. Method of study We inhibited eIF 5A and Ca 2+ /calmodulin‐dependent protein kinase 1D ( CAMK 1D) expression in HTR 8 cells using RNA interference. The effects of eIF 5A and CAMK 1D on HTR 8 cells were investigated using real‐time polymerase chain reaction, Western blotting, flow cytometry, cell transfection assays, cell migration assays, and terminal deoxynucleotidyl transferase dUTP nick‐end labeling. Results eIF 5A inhibition decreased CAMK 1D expression, proliferation, migration, and invasion, but upregulated apoptosis, in HTR 8 cells. Conclusion Cross‐talk between eIF 5A and CAMK 1D enhances proliferation, migration, and invasion, but inhibits apoptosis, in trophoblasts.