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Lipopolysaccharide and cAMP modify placental calcitriol biosynthesis reducing antimicrobial peptides gene expression
Author(s) -
OlmosOrtiz Andrea,
GarcíaQuiroz Janice,
Avila Euclides,
CaldiñoSoto Felipe,
Halhali Ali,
Larrea Fernando,
Díaz Lorenza
Publication year - 2018
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12841
Subject(s) - cathelicidin , calcitriol , antimicrobial peptides , biology , downregulation and upregulation , beta defensin , endocrinology , medicine , biochemistry , antimicrobial , vitamin d and neurology , microbiology and biotechnology , gene
Problem Calcitriol, the hormonal form of vitamin D 3 ( VD ), stimulates placental antimicrobial peptides expression; nonetheless, the regulation of calcitriol biosynthesis in the presence of bacterial products and its consequence on placental innate immunity have scarcely been addressed. Method of study We investigated how some bacterial products modify placental VD metabolism and its ability to induce antimicrobial peptides gene expression. Results Cultured human trophoblasts biosynthesized calcitriol only in the presence of its precursor calcidiol, a process that was inhibited by cyclic‐ AMP but stimulated by lipopolysaccharide ( LPS ). Intracrine calcitriol upregulated cathelicidin, S100A9, and β‐defensins ( HBD s) gene expression, while LPS further stimulated HBD 2 and S100A9 . Unexpectedly, LPS significantly repressed cathelicidin basal mRNA levels and drastically diminished calcidiol ability to induce it. Meanwhile, cyclic‐ AMP , which is used by many microbes to avoid host defenses, suppressed calcitriol biosynthesis, resulting in significant inhibition of most VD ‐dependent microbicidal peptides gene expression. Conclusion While LPS stimulated calcitriol biosynthesis, cyclic‐ AMP inhibited it. LPS downregulated cathelicidin mRNA expression, whereas cyclic‐ AMP antagonized VD ‐dependent‐upregulation of most antimicrobial peptides. These findings reveal LPS and cyclic‐ AMP involvement in dampening placental innate immunity, highlighting the importance of cyclic‐ AMP in the context of placental infection and suggesting its participation to facilitate bacterial survival.