Premium
The pregnancy hormone human chorionic gonadotropin differentially regulates plasmacytoid and myeloid blood dendritic cell subsets
Author(s) -
Sauss Karoline,
Ehrentraut Stefanie,
Zenclussen Ana Claudia,
Schumacher Anne
Publication year - 2018
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12837
Subject(s) - human chorionic gonadotropin , hormone , secretion , biology , myeloid , dendritic cell , endocrinology , medicine , immune system , gonadotropin , immune tolerance , trophoblast , regulator , placenta , immunology , fetus , pregnancy , gene , biochemistry , genetics
Problem Dendritic cells ( DC s) are critically involved in fetal fate due to their capability to promote either immunity or tolerance to foreign fetal antigens. Our study aimed to investigate whether the hormone human chorionic gonadotropin ( hCG ) affects different peripheral blood DC ( PBDC s) subsets. Method of study PBDC s were isolated from non‐pregnant women, stimulated and co‐cultured with hCG ‐producing JEG ‐3 or non hCG ‐producing SWAN ‐71 trophoblasts as well as with two different hCG preparations. The total and mature number of each PBDC subset were assessed. Additionally, the secretion of cytokines was determined in the presence or absence of hCG . Results We found no significant effects; neither from trophoblasts nor from the hCG preparations on PBDC cellularity. JEG ‐3 cells and both hCG preparations significantly hampered the maturation of MDC 1, while SWAN ‐71 cells did not provoke any changes on this regard. We observed an altered ability of PBDC s to secrete cytokines in the presence of both trophoblasts and both hCG preparations. Conclusion Our data propose that hCG is not a key regulator of cellular alterations within PBDC s. However, under inflammatory conditions, hCG seems to keep the delicate balance between PDC and MDC and retains a tolerogenic MDC 1 profile and this may contribute to tolerance maintenance.