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Application of computer‐aided sperm analysis (CASA) for detecting sperm‐immobilizing antibody
Author(s) -
Wakimoto Yu,
Fukui Atsushi,
Kojima Teruhito,
Hasegawa Akiko,
Shigeta Minoru,
Shibahara Hiroaki
Publication year - 2018
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12814
Subject(s) - sperm , andrology , antibody , semen analysis , biology , chemistry , medicine , immunology , infertility , pregnancy , genetics
Problem Since the 1970s, anti‐sperm antibodies have been studied as a pathogenic factor contributing to infertility. The complement‐dependent sperm‐immobilization test (SIT) and quantitative SIT have been used as effective tools for detecting anti‐sperm antibodies in clinical settings. These tests have been carried out traditionally by manually counting the number of motile sperm through eye estimation. Method of study In this study, we developed a novel method using computer‐aided sperm analysis. The results were compared with those obtained by the traditional method. Results The results were identical and 25 of 78 samples tested were positive and 53 samples were negative for sperm‐immobilizing (SI) antibodies based on both methods. For SI‐positive samples, the values of SI 50 obtained using the two methods correlated closely with high co‐efficiency. Conclusion Using the novel method, manually counting the number of motile spermatozoa becomes unnecessary. The novel method presented here will increase the objectivity and convenience of using the SIT as a clinical indicator.