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Impaired Treg and NK cells profile in overweight women with gestational diabetes mellitus
Author(s) -
Lobo Thalita Frutuoso,
Borges Camila de Moraes,
Mattar Rosiane,
Gomes Caio Perez,
Angelo Ana Geisa Santos,
Pendeloski Karen Priscilla Tezotto,
Daher Silvia
Publication year - 2018
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12810
Subject(s) - granzyme b , gestational diabetes , immune system , foxp3 , overweight , medicine , flow cytometry , immunology , perforin , peripheral blood mononuclear cell , diabetes mellitus , endocrinology , obesity , gestation , pregnancy , cd8 , biology , biochemistry , in vitro , genetics
Problem Maternal obesity is frequently associated with gestational diabetes mellitus ( GDM ), and immunological mechanisms seem to be involved in the physiopathology of these conditions. The aim of this study was to characterize the profile of immune cells in peripheral blood of overweight women with GDM . Method of Study This case‐control study included 27 glucose‐tolerant (controls) and 31 GDM overweight pregnant women. Flow cytometry was used to assess the number of regulatory T cells (Treg) and natural killer ( NK ) cells in the peripheral blood. In addition, the expression of IL ‐10, TGF ‐B, and TNF ‐A in Treg and expression of IFN ‐G, TNF ‐A, granzyme, and perforin in NK cells were analyzed. Results GDM patients had significantly lower frequency of TCD 4 + CD 25 bright and TCD 4 + CD 25 + FOXP 3 high cells, higher production of TNF ‐A by Treg cells and higher percentage of NKCD 16 + 56 dim cells than the controls. Conclusion The association between obesity and GDM is a condition where it is observed impaired Treg and NK cells profile, findings that seem to be related with the development of IR and inflammation.