Premium
Sludge reflects intra‐amniotic inflammation with or without microorganisms
Author(s) -
Yoneda Noriko,
Yoneda Satoshi,
Niimi Hideki,
Ito Masami,
Fukuta Kaori,
Ueno Tomohiro,
Ito Mika,
Shiozaki Arihiro,
Kigawa Mika,
Kitajima Isao,
Saito Shigeru
Publication year - 2018
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12807
Subject(s) - chorioamnionitis , amniotic fluid , microorganism , gestation , inflammation , andrology , microbiology and biotechnology , biology , medicine , gastroenterology , pregnancy , bacteria , fetus , genetics
Problem To investigate whether amniotic fluid ( AF ) “sludge” in patients with preterm labor ( PTL ) with intact membranes is related to intra‐amniotic infection or inflammation. Method of study 105 PTL patients before 29 weeks’ gestation were enrolled. AF “sludge” was evaluated by transvaginal sonography. Microorganisms were identified in AF by our newly established PCR method using a eukaryote‐made thermostable DNA polymerase. Results AF “sludge” was present in 18.1% (19/105) of patients. The results obtained in the AF “sludge” group vs the no “sludge” group were as follows: (i) a similar positive rate of microorganisms in AF by PCR , 31.6% (6/19) vs 38.4% (33/86); (ii) a higher level of AF interleukin‐8, 15.2 (0.2‐381.5) ng/mL vs 5.8 (0.1‐413.7) ng/ mL ; P = .005); and (3) a higher frequency of histological chorioamnionitis, 52.6% (10/19) vs 23.3% (20/86); P = .010. Conclusion The presence of AF “sludge” is related to intra‐amniotic inflammation with or without microorganisms.