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Decidual CD 68 + HLA ‐ DR + CD 163 − M1 macrophages increase in miscarriages with normal fetal chromosome
Author(s) -
Shimada Shigeki,
Ebina Yasuhiko,
Iijima Norifumi,
Deguchi Masashi,
Yamada Hideto
Publication year - 2018
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12791
Subject(s) - fetus , andrology , microbiology and biotechnology , flow cytometry , x chromosome , in utero , biology , immunology , pregnancy , medicine , genetics , gene
Problem Is an abnormal increase or decrease of M1/M2 macrophages observed in the deciduae of miscarriages with normal fetal chromosome ( MN )? Methods of study Deciduae of 18 MN and 26 miscarriages with abnormal fetal chromosome ( MA ) were obtained. Additionally, deciduae from 15 women whose pregnancies ended in induced abortions ( IA ) and endometriums at the mid‐luteal phase from 19 non‐pregnant women endomeriums of mid‐luteal phases ( EM ) were obtained. Macrophages were analyzed by flow cytometry using monoclonal antibodies for CD 68, HLA ‐ DR , and CD 163. Results M1 macrophages, defined as CD 68 + HLA ‐ DR + CD 163 − cells, increased in MN compared with MA or IA . M2 macrophages, defined as CD 68 + HLA ‐ DR − CD 163 + cells, increased in the deciduae of MA and IA compared with EM . However, this increase was not observed in the deciduae of MN . Conclusion Our findings of phenotypic characters of decidual macrophages in MN provide additional evidence that M2 polarization is favorable for the maintenance of early stages of pregnancy.