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The immune profile induced is crucial to determine the effects of immunocastration over gonadal function, fertility, and GnRH‐I expression
Author(s) -
Siel Daniela,
Loaiza Alexandra,
Vidal Sonia,
Caruffo Mario,
Paredes Rodolfo,
Ramirez Galia,
Lapierre Lisette,
Briceño Cristóbal,
Pérez Oliver,
Sáenz Leonardo
Publication year - 2018
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12772
Subject(s) - immune system , endocrinology , immunogenicity , biology , gonadotropin releasing hormone , fertility , hormone , medicine , luteal phase , immunology , luteinizing hormone , population , environmental health
Problem Immunocastration or vaccination against the GnRH‐I hormone is a promising alternative to reproductive control in different animal species. Given the low immunogenicity of this hormone, the use of adjuvants becomes necessary. Method of Study This study evaluated the effects of three adjuvants that induce different immune response profiles over gonadal function, fertility, and expression of GnRH‐I. Female mice (n = 6) were vaccinated at days 1 and 30 with a recombinant antigen for immunocastration and different adjuvants that induced preferentially Th1/Th2, Th2, and Th1 immune profiles. Results Th1/Th2 response is the most efficient to block reproductive activity in vaccinated animals, reducing the number of luteal bodies and pre‐ovulatory follicles. Th2 and Th1/Th2 responses induced an increase in GnRH‐I at the hypothalamus. Conclusion The immune profile induced by different adjuvants is essential on the effects over fertility, gonadal function, and hypothalamic GnRH‐I expression in immunocastrated animals.