Premium
Rapid and simple detection of Ureaplasma species from vaginal swab samples using a loop‐mediated isothermal amplification method
Author(s) -
Fuwa Kazumasa,
Seki Mitsuko,
Hirata Yoshiyasu,
Yanagihara Itaru,
Nakura Yukiko,
Takano Chika,
Kuroda Kazumichi,
Hayakawa Satoshi
Publication year - 2018
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12771
Subject(s) - loop mediated isothermal amplification , ureaplasma urealyticum , ureaplasma , microbiology and biotechnology , biology , polymerase chain reaction , gene , genetics , dna , mycoplasma
Problem Ureaplasma species occasionally cause chorioamnionitis and premature labor. We developed a novel assay employing a loop‐mediated isothermal amplification ( LAMP ) method to detect Ureaplasma parvum and Ureaplasma urealyticum . Method of study Loop‐mediated isothermal amplification primers were designed to amplify Ureaplasma ‐specific ureaseB genes. Four U. parvum strains, 5 U. urealyticum strains and 14 reference bacterial species were evaluated. Forty‐six vaginal swab samples were analyzed by LAMP , culture, and PCR . Results Our LAMP primers were specific to each species and had no cross‐reaction. Of 46 clinical specimens, the sensitivity, specificity, and positive and negative predictive values of the LAMP method were 100% (12/12), 100% (34/34), 100% (12/12), and 100% (34/34), respectively, whereas those of PCR were 66.7% (8/12), 100% (34/34), 100% (8/8), and 89.5% (34/38), respectively, compared to culture‐based detection. Conclusion The LAMP detection method outperformed the culture and PCR methods. Early detection enables appropriate antibiotic selection for improved prenatal outcomes.