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Distinct peripheral vs mucosal T‐cell phenotypes in chlamydia‐infected women
Author(s) -
Ogendi Brian M. O.,
Bakshi Rakesh K.,
Sabbaj Steffanie,
Brown LaDraka',
Lee Jeannette Y.,
Kapil Richa,
Geisler William M.
Publication year - 2017
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12768
Subject(s) - chlamydia , phenotype , flow cytometry , immunology , biology , peripheral blood mononuclear cell , homing (biology) , t cell , chemokine , immune system , cell , chlamydia trachomatis , chemokine receptor , in vitro , gene , ecology , biochemistry , genetics
Problem Differences in circulating (peripheral) and mucosal T‐cell phenotypes in chlamydia‐infected women remain largely unknown. Method of study Thirteen paired mononuclear cell specimens from blood and cervicovaginal lavages collected from chlamydia‐infected women were stained and analyzed using ten‐color cell surface flow cytometry for T‐cell distribution, activation status, homing, and T helper (Th)‐associated chemokine receptors ( CKR s). Results A higher proportion of genital mucosal T‐cells were activated ( CD 38 + HLA ‐ DR + ) and expressed CCR 5 and Th1‐associated CKR CXCR 3 + CCR 5 + compared to peripheral T‐cells, but a lower proportion of mucosal T‐cells expressed homing CKR CCR 7, Th‐2 associated CKR CCR 4, and CXCR 3 + CCR 4 + for both T‐cell subsets. Conclusion T‐cell phenotypes differed in the peripheral vs genital mucosa compartments in chlamydia‐infected women. As chlamydia infects mucosal epithelial cells, the finding of a higher frequency of activated T‐cells and Th‐1 phenotypes in the mucosa likely reflects an adaptive immune response to infection.