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Reduced CD 200 expression is associated with altered Th1/Th2 cytokine production in placental trophoblasts from preeclampsia
Author(s) -
Xu Jie,
Gu Yang,
Sun Jingxia,
Zhu Hui,
Lewis David F.,
Wang Yuping
Publication year - 2018
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12763
Subject(s) - trophoblast , preeclampsia , cytokine , immunostaining , placenta , andrology , fetal membrane , biology , transfection , downregulation and upregulation , endocrinology , medicine , immunology , fetus , pregnancy , gene , immunohistochemistry , genetics , biochemistry
Problem To determine if altered trophoblast CD 200 and CD 200R expressions promote inflammatory cytokine production in preeclamptic placentas. Methods of study Placental tissue CD 200 and CD 200R expressions were determined by immunostaining. Tissue sections from first‐, second‐, and third‐trimester, normal term, and preeclamptic placentas were used. CD 200 and CD 200R expressions and cytokine production of TNF α, sTNFR 1, INF γ, IL ‐4, IL ‐6, IL ‐8, and IL ‐10 were determined in trophoblasts from normal and preeclamptic placentas and in normal trophoblasts transfected with CD 200 si RNA . Results CD 200, but not CD 200R, expression was significantly reduced in trophoblasts from preeclamptic compared to normal placentas. Trophoblast from preeclamptic placentas and trophoblast transfected with CD 200 si RNA produced significantly more TNF α, sTNFR 1, IL ‐6, and IL ‐8, but significantly less IL ‐10, than trophoblasts from normal control placentas. Conclusion Downregulation of CD 200 expression resulted in an imbalance of increased Th1 cytokine and decreased Th2 cytokine production in placental trophoblasts in preeclampsia.