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Identification of genes dysregulated by elevation of micro RNA ‐210 levels in human trophoblasts cell line, Swan 71
Author(s) -
Ahn Sejin,
Jeong Eunbee,
Min Jae Woong,
Kim Eunhee,
Choi Sun Shim,
Kim Chong Jae,
Lee DeugChan
Publication year - 2017
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12722
Subject(s) - preeclampsia , microarray , biology , transcriptome , trophoblast , gene , microarray analysis techniques , gene expression , placenta , endocrinology , andrology , medicine , fetus , pregnancy , genetics
Problem Preeclampsia is a serious pregnancy disorder characterized by gestational hypertension and proteinuria. miR‐210 is significantly overexpressed in the placentas of preeclampsia patients. Method of study Swan 71 cells, first‐trimester human trophoblastic cell line, were transfected with hsa‐miR‐210‐3p oligonucleotides by electroporation. Altered transcriptome was analyzed using microarray technique. Differentially expressed genes ( DEG s) were clustered into Gene Ontology annotation biological processes. The extent of physical interaction between miR‐210 and IGFBP 3 mRNA was assessed via ribonucleoprotein immunoprecipitation. Results Microarray analysis showed 408 DEG s by elevated levels of miR‐210 in Swan 71 cells. These genes were enriched in several biological processes involved in the pathogenesis of preeclampsia. IGFBP 3 , a gene associated with preeclampsia pathophysiology, was validated as a target gene of miR‐210. Conclusion We have demonstrated that elevated miR‐210 levels in human trophoblast alter the expression profile of known preeclampsia‐associated genes, and of gene targets involved in various biological processes essential to preeclampsia progression.