Premium
Detection of dendritic cells and related cytokines in follicular fluid of patients with polycystic ovary syndrome
Author(s) -
Zhang Tao,
Tian Fuying,
Huo Ran,
Tang Aifa,
Zeng Yong,
Duan YongGang
Publication year - 2017
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12717
Subject(s) - polycystic ovary , follicular fluid , medicine , folliculogenesis , endocrinology , flow cytometry , follicular phase , infertility , ovary , follicle , tumor necrosis factor alpha , ovarian follicle , andrology , oocyte , biology , pregnancy , immunology , embryo , insulin , insulin resistance , genetics , lactation , microbiology and biotechnology
Problem The presence of dendritic cells (DCs) and associated cytokines in follicular fluid (FF) from patients with polycystic ovary syndrome (PCOS) remains unknown. Methods of study FF was collected from PCOS patients and patients with severe male factor infertility (control) at the day of transvaginal oocyte retrieval. Phenotypes of DC were detected by flow cytometry, and TNF‐α, IL‐6, IL‐10, and IL‐23 were assessed by ELISA. Results A significant decrease in the percentage of DC was found in patients with PCOS (16.22±5.5%) compared with control (21.27±5.5%, P <.01). E 2 on the day of hCG administration was correlated positively with the mean fluorescence intensity of HLA‐DR ( r =.75, P <.01) and reversely correlated with the concentration of TNF‐α in FF ( r =−.69, P <.01). The level of TNF‐α, IL‐6, and IL‐10 increased significantly but IL‐23 decreased in FF from patients with PCOS. Conclusion The decrease of DC and disturbance of associated cytokines in FF from PCOS patients indicates a disorder of immunological microenvironment of the ovarian follicle, which might be involved in the dysfunction of folliculogenesis.