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Expression profile of heat shock proteins in placental tissues of patients with preterm prelabor rupture of membranes and spontaneous preterm labor with intact membranes
Author(s) -
Dvorakova Lenka,
Ivankova Katarina,
Krofta Ladislav,
Hromadnikova Ilona
Publication year - 2017
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12698
Subject(s) - downregulation and upregulation , hsp27 , heat shock protein , hsp70 , medicine , andrology , hsp60 , gestational age , pregnancy , endocrinology , biology , gene , genetics
Problem Investigating the stress response in the central cotyledon zone of placental tissue in pregnancies with PPROM , PTB , and at term in labor. Method of study Gene expression of Hsp27, Hsp60, Hsp70, Hsp90, and Hsp BP 1 was compared between these particular groups. Correlation between variables including Hsp gene expression in placental tissue and the gestational age at delivery, WBC count at admission, and serum levels of CRP at admission in patients with PPROM and PTB was determined. Results Both PPROM and PTB pregnancies were associated with altered Hsp gene expression profile. While PPROM and PTB always induced upregulation of Hsp27 and Hsp60, downregulation of Hsp70 and Hsp BP 1 was present entirely in patients with PPROM . Hsp BP 1 expression profile was also able to differentiate between PPROM and PTB pregnancies. The highest mRNA levels of Hsp60 and Hsp70 were detected in PTB pregnancies with elevated CRP levels at admission. Some of the examined Hsp displayed increased expression with advancing gestational age in both groups ( PPROM : Hsp27, Hsp70, and Hsp90; and PTB : Hsp27). Conclusion Upregulation of Hsp27 is a common phenomenon shared between pregnancies affected with PTB and PPROM . On the other hand, downregulation of Hsp70 and Hsp BP 1 represents a unique feature of PPROM .