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Soy isoflavones enhance β‐defensin synthesis and secretion in endometrial epithelial cells with exposure to TLR 3 agonist polyinosinic‐polycytidylic acid
Author(s) -
Srisomboon Yotesawee,
Poonyachoti Sutthasinee,
Deachapunya Chatsri
Publication year - 2017
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12694
Subject(s) - genistein , daidzein , secretion , innate immune system , isoflavones , endometrium , endocrinology , downregulation and upregulation , chemistry , medicine , defensin , microbiology and biotechnology , biology , biochemistry , receptor , gene
Problem β‐defensins are important innate chemical barriers that protect the endometrium from pathogen invasion. The effects of soy isoflavones, genistein and daidzein, on the expression and secretion of porcine β‐defensins ( PBD ) in endometrial epithelial cells were investigated under normal or poly I:C‐stimulated conditions. Method of study Primary cultured porcine endometrial epithelial ( PE ) cells were pretreated with genistein or daidzein followed by poly I:C inoculation. During treatment, the culture media were analyzed for PBD 1‐4 secretion by ELISA and the total RNA for PBD gene expression by quantitative RT ‐ PCR . Results Porcine endometrial epithelial cells constitutively expressed PBD 1‐4 and secreted PBD ‐1, PBD ‐2, and PBD ‐4. Genistein and daidzein enhanced PBD ‐2 expression and PBD ‐2 and PBD ‐3 secretion. These compounds also potentiated PBD‐2 and PBD ‐3 expression and secretion which were upregulated by poly I:C. Conclusion Soy isoflavones, genistein and daidzein, could be potentially used for promoting the innate host defense of endometrium against infection.