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Activation of NOD ‐1/ JNK / IL ‐8 signal axis in decidual stromal cells facilitates trophoblast invasion
Author(s) -
Ryu Byung Jun,
Han Jae Won,
Kim Ryang Hee,
Yun Sohyun,
Kim Tae Hyun,
Hur Sung Eun,
Kim Chul Jung,
Lee Sung Ki
Publication year - 2017
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12672
Subject(s) - trophoblast , decidua , microbiology and biotechnology , stromal cell , signal transduction , biology , secretion , cytokine , chemistry , placenta , immunology , endocrinology , cancer research , fetus , pregnancy , genetics
Decidual stromal cells ( DSC s) are known to regulate trophoblast invasion via unveiled mechanism yet. And nucleotide‐binding oligomerization domain‐containing protein 1 ( NOD 1) may influence on this DSC ‐trophoblast interaction. We investigated the mechanism underlying the DSC ‐mediated regulation of trophoblast invasion and the effect of NOD 1 on their cross talk. Using human primary DSC s, BeWo cell invasion was measured. Cytokine secretion and MAP kinase signaling were examined in DSC s following treatment with NOD 1 agonist, Tri‐ DAP . DSC s secreted IL ‐8 and increased trophoblast invasion. Tri‐ DAP further increased IL ‐8 secretion from DSC s via JNK pathway and facilitated both MMP ‐2 production and trophoblast invasion compared with control. Upon cotreatment of IL ‐8 and anti‐ IL ‐8 antibody to BeWo cells, the number of invading trophoblasts and MMP ‐2 production decreased significantly. These results suggest that IL ‐8 from DSC s may play a role to increase the invasiveness of trophoblast cells into the decidua via NOD 1/ JNK pathway.