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Molecular displaying of differential immunoresponse to various infections of amniotic epithelia
Author(s) -
Nemr Waleed,
Bashandy Monem,
Araby Eman,
Khamiss Omaima
Publication year - 2017
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12662
Subject(s) - biology , innate immune system , defensin , antimicrobial peptides , antimicrobial , gene , gene expression , polymerase chain reaction , beta defensin , microbiology and biotechnology , differential display , downregulation and upregulation , pathogen , rna , amniotic fluid , amnion , immune system , immunology , genetics , fetus , pregnancy
Problem Molecular displaying for the interaction of innate immune cells against pathogen is important for knowing their defense mechanism. This study aimed to visualize the differential gene expression profiles of amniotic epithelium, in response to various infections, using simple reverse transcription polymerase chain reaction (RT‐PCR) techniques. Method of study Antimicrobial activity of human amniotic epithelial cells (HAECs) was verified against three different models of pathogenic microorganisms. RNA was extracted from infected and non‐infected cells. Transcripts were visualized by two methods of RT‐PCR; one of them targets β‐defensin 2 (BDEF2). The other is a novel design method of RNA fingerprinting based on differential length amplification of transcripts (DifLAT) to polymorphism more than 69 coding sequences for antimicrobial proteins. Results The semiquantitative gel analysis indicated that BDEF2 was upregulated during all infections. DifLAT experiment visualized different patterns of HAECs transcripts for each case of infection. Conclusion This study proved that HAECs uses alternative gene expression profiles for fighting different pathogens.