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Palmitic acid stimulates interleukin‐8 via the TLR4/NF‐κB/ROS pathway and induces mitochondrial dysfunction in bovine oviduct epithelial cells
Author(s) -
Ohtsu Ayaka,
Tanaka Hazuki,
Seno Kotomi,
Iwata Hisataka,
Kuwayama Takehito,
Shirasuna Koumei
Publication year - 2017
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12642
Subject(s) - oviduct , mitochondrial ros , reactive oxygen species , endocrinology , biology , medicine , corpus luteum , palmitic acid , mitochondrion , microbiology and biotechnology , chemistry , fatty acid , biochemistry , hormone
Problem We investigated the effect of palmitic acid ( PA ), a major saturated fatty acid in NEFA , on bovine oviduct epithelial cells ( OEC s) during in vitro cell culture. Method of study Bovine oviductal tissues ipsilateral to the corpus luteum were collected 1‐3 days after ovulation; the OEC s were isolated and cultured. Results PA increased lipid accumulation and activated caspase‐3 in OEC s, resulting in decreased cell proliferation. PA also stimulated the secretion of inflammatory cytokine interleukin ( IL )‐8 depending on TLR 4, NF ‐κB activation, and reactive oxygen species ( ROS ) production. Moreover, PA induced mitochondrial dysfunction, including mitochondrial fission, ATP production, and mitochondrial ROS production. It also increased levels of LC 3 and p62 proteins, suggesting autophagy induction in OEC s. Conclusion We suggest that bovine OEC s recognize an excessive increase in endogenous and sterile danger signals, such as PA , which may contribute to chronic oviductal inflammation, resulting in infertility associated with oviductal dysfunction.