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Time course analysis of RNA quality in placenta preserved by RNA later or flash freezing
Author(s) -
Martin Nicole M.,
Cooke Katherine M.,
Radford Caitlin C.,
Perley Lauren E.,
Silasi Michelle,
Flannery Clare A.
Publication year - 2017
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12637
Subject(s) - decidua , housekeeping gene , placenta , rna , andrology , biology , housekeeping , gene expression , gene , medicine , pregnancy , biochemistry , fetus , genetics
Problem Preservation of biospecimen quality is critical to accurately and reliably assessing genes and proteins. We evaluated the effect of preparation method and storage duration on RNA quality in placenta and decidua. Method of study Aliquots of nine placentas and decidua were placed in RNA later ® (RL) or flash frozen (FF) within 30 minutes of delivery. RNA was extracted immediately (baseline) and from matched samples stored at −80°C for 1 and 8‐10 months. RNA Integrity Number (RIN) and housekeeping gene expression were quantified. Results At both time points, RL placenta had RIN and housekeeping gene Ct values similar to baseline. However, FF placenta had significantly lower RIN and higher Ct values at 1 and 8‐10 months. In RL and FF decidua, RIN was unchanged from baseline. Conclusion We found RNA later more effectively and consistently preserved placenta, compared to flash freezing. However, for decidua, which is less dense than placenta, both modes yielded comparable RNA integrity over time.