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Vitamin D3 pretreatment protects against lipopolysaccharide‐induced early embryo loss through its anti‐inflammatory effects
Author(s) -
Zhou Yan,
Chen YuanHua,
Fu Lin,
Yu Zhen,
Xia MiZhen,
Hu XiaoGuang,
Wang Hua,
Xu DeXiang
Publication year - 2017
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12620
Subject(s) - endocrinology , lipopolysaccharide , medicine , embryo , andrology , vitamin , proinflammatory cytokine , immunology , inflammation , biology , microbiology and biotechnology
Problem Increasing evidence demonstrates that inflammatory cytokines are involved in LPS ‐induced adverse pregnant outcomes including early embryo loss. Vitamin D3 (VitD3) has anti‐inflammatory activity. We aimed to investigate the effects of vitamin D3 (VitD3) on LPS ‐induced early embryo loss in mice. Method of study All pregnant mice except controls were intraperitoneally (ip) injected with LPS on GD 7. In VitD3 alone and LPS +VitD3 groups, pregnant mice were pretreated with VitD3 by gavage daily from GD 5 to GD 7. Results LPS caused 62.5% pregnant mice with early embryo loss. Interestingly, the rate of abortion dropped to 14.3% when pregnant mice were pretreated with VitD3. Additional experiment showed that VitD3 significantly attenuated LPS ‐evoked elevation on TNF ‐α, IFN ‐γ, MIP ‐2, and nitrate plus nitrite in maternal serum. In addition, VitD3 alleviated LPS ‐induced COX ‐2 expression in the decidua and attenuated the elevation of PGF 2α in maternal serum. Although VitD3 had no effect on IL ‐10 in maternal serum, it induced further elevation of serum IL ‐10 level in LPS ‐treated mice. Further analysis showed that VitD3 activated VDR signaling, simultaneously inhibited LPS ‐induced nuclear translocation of NF ‐κB p65 subunits in the decidua. Conclusions VitD3 protects mice from LPS ‐induced early embryo loss at least partially through its anti‐inflammatory effects.