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Neutrophil extracellular traps in acute chorioamnionitis: A mechanism of host defense
Author(s) -
GomezLopez Nardhy,
Romero Roberto,
Leng Yaozhu,
GarciaFlores Valeria,
Xu Yi,
Miller Derek,
Hassan Sonia S.
Publication year - 2017
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12617
Subject(s) - neutrophil extracellular traps , mechanism (biology) , extracellular , defence mechanisms , host (biology) , chorioamnionitis , immunology , biology , microbiology and biotechnology , chemistry , medicine , inflammation , pregnancy , ecology , fetus , biochemistry , genetics , philosophy , epistemology , gene
Problem Neutrophil extracellular traps ( NET s) were recently described as a mechanism for microbial killing in the amniotic cavity of women with intra‐amniotic infection. Such a clinical condition can result in acute chorioamnionitis, a placental lesion characterized by the infiltration of maternal neutrophils in the chorioamniotic membranes. Herein, we investigated whether these infiltrating neutrophils form NET s in the chorioamniotic membranes from women who underwent spontaneous term or preterm labor with acute chorioamnionitis. Method of study Chorioamniotic membrane samples were collected from women who underwent spontaneous term or preterm labor with acute chorioamnionitis (n=10 each). Controls included chorioamniotic membrane samples from women who delivered at term or preterm with or without labor in the absence of acute chorioamnionitis (n=10 each). NET s were visualized and semiquantified in the chorioamniotic membranes by using antibodies against neutrophil elastase and histone H3 in combination with DAPI staining. Results Neutrophil extracellular traps were abundant in the chorioamniotic membranes from women who underwent spontaneous term or preterm labor with acute chorioamnionitis. NET s were rarely found, or not visualized at all, in the chorioamniotic membranes from women who delivered at term or preterm with or without labor in the absence of acute chorioamnionitis. Conclusion Neutrophil extracellular traps are abundant in the chorioamniotic membranes from women who underwent spontaneous term or preterm labor with acute chorioamnionitis. These findings suggest that chorioamniotic neutrophils can form NET s as a mechanism of host defense against infection or danger signals.