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In vivo T‐cell activation by a monoclonal α CD 3ε antibody induces preterm labor and birth
Author(s) -
GomezLopez Nardhy,
Romero Roberto,
ArenasHernandez Marcia,
Ahn Hyunyoung,
Panaitescu Bogdan,
VadilloOrtega Felipe,
SanchezTorres Carmen,
Salisbury Katherine S.,
Hassan Sonia S.
Publication year - 2016
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12562
Subject(s) - isotype , monoclonal antibody , cd3 , fetus , antibody , medicine , andrology , immunology , biology , endocrinology , pregnancy , cd8 , immune system , genetics
Problem Activated/effector T cells seem to play a role in the pathological inflammation associated with preterm labor. The aim of this study was to determine whether in vivo T‐cell activation by a monoclonal α CD 3ε antibody induces preterm labor and birth. Method of study Pregnant B6 mice were intraperitoneally injected with a monoclonal α CD 3ε antibody or its isotype control. The gestational age, the rates of preterm birth and pup mortality at birth as well as the fetal heart rate and umbilical artery pulsatility index were determined. Results Injection of a monoclonal α CD 3ε antibody led to preterm labor/birth (α CD 3ε 83 ± 16.97% [10/12] vs isotype 0% [0/8]) and increased the rate of pup mortality at birth (α CD 3ε 87.30 ± 8.95% [77/85] vs isotype 4.91 ± 4.34% [3/59]). In addition, injection of a monoclonal α CD 3ε antibody decreased the fetal heart rate and increased the umbilical artery pulsatility index when compared to the isotype control. Conclusion In vivo T‐cell activation by a monoclonal α CD 3ε antibody in late gestation induces preterm labor and birth.