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Effects of bilateral vasectomy on the interleukin 1 system in mouse epididymis
Author(s) -
Oh Yeong Seok,
Na Eun Ju,
Gye Myung Chan
Publication year - 2016
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12548
Subject(s) - epididymis , interleukin 1 receptor antagonist , interleukin 10 , interleukin 1 receptor, type i , medicine , endocrinology , interleukin , receptor antagonist , tumor necrosis factor alpha , receptor , interleukin 6 , antagonist , biology , cytokine , andrology , sperm
Problem Regional difference in the inflammatory response in vasectomized ( VAX ) epididymis remains unraveled. Method of study Epididymal expression of interleukin 1α ( IL 1α), IL 1β, IL 1 receptor antagonist ( IL 1ra), IL 1 receptor 1 ( IL 1R1), IL 6, IL 10, tumor necrosis factor α ( TNF ‐α), and transforming growth factor‐β1 ( TGF ‐β1) was examined in bilateral VAX mice by real‐time RT ‐ PCR and immunohistochemistry. Results IL 1α, IL 1β, IL 1ra, and IL 1R1 were expressed in the epididymal epithelia of normal mice. Following VAX , IL 1R1 mRNA was not changed, but TNF ‐α , IL 10 , IL 1α , IL 1β , and IL 1ra mRNA were significantly upregulated in whole epididymis, whereas IL 6 and TGF ‐β1 mRNA were significantly increased in corpus and cauda but not in caput epididymis. Consistently, IL 1α, IL 1β, and IL 1ra immunoreactivities were visibly increased in epididymis following VAX . Conclusion Following VAX , IL 1α, IL 1β, IL 1ra, IL 10, and TNF ‐α may mediate immune reaction in whole epididymis, whereas IL 6 and TGF ‐β1 may mediate regionally different immune response primarily in the lower part of epididymis.