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Divergence of helper, cytotoxic, and regulatory T cells in the decidua from miscarriage
Author(s) -
Ebina Yasuhiko,
Shimada Shigeki,
Deguchi Masashi,
Maesawa Yoko,
Iijima Norifumi,
Yamada Hideto
Publication year - 2016
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12546
Subject(s) - decidua , cytotoxic t cell , foxp3 , flow cytometry , fetus , recurrent miscarriage , karyotype , biology , andrology , miscarriage , immunology , medicine , immune system , microbiology and biotechnology , pregnancy , chromosome , placenta , genetics , gene , in vitro
Problem The aim of this prospective study was to evaluate phenotypic differences of helper T (Th), cytotoxic T (Tc), and regulatory T (Treg) cells in the deciduae of missed miscarriage with a normal chromosome karyotype of a fetus ( MN ) and missed miscarriage with an abnormal chromosome karyotype of a fetus ( MA ). Methods of study The decidua of 19 MN and 28 MA was obtained. Additionally, the decidua of 15 induced abortion ( IA ) and the endometrium of 19 non‐pregnant women ( EM ) were obtained. IFN ‐γ + , IL ‐17 + , CD 25 high Foxp3 + cells in CD 4 + (Th) cells, and IFN ‐γ + cells in CD 8 + (Tc) cells were evaluated by flow cytometry. Results The percentages of IFN ‐γ + Tc and CD 4 + CD 25 high Foxp3 + (Treg) cells in MN were significantly increased as compared with MA and IA . The percentage of IFN ‐γ + Th in MN was increased as compared with IA . Conclusion Activation of IFN ‐γ + Tc and Treg cells in the decidua might be associated with the pathophysiology underlying MN .