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Predictive value of maternal serum NF‐κB p65 and sTREM‐1 for subclinical chorioamnionitis in premature rupture of membranes
Author(s) -
Wang Fan,
Wang Yuhuan,
Wang Rongyue,
Qiu Haifan,
Chen Lulu
Publication year - 2016
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12543
Subject(s) - chorioamnionitis , prom , premature rupture of membranes , subclinical infection , medicine , cord blood , immunology , gastroenterology , andrology , obstetrics , fetus , pregnancy , biology , genetics
Problem The aim of this study was to investigate the levels of nuclear factor kappa B‐p65 (NF‐κB p65) and soluble triggering receptor expressed on myeloid cells‐1 (sTREM‐1) in maternal blood with premature rupture of membranes (PROM) and to assess their values for prediction of subclinical chorioamnionitis. Method NF‐κB p65 and sTREM‐1 levels were measured in maternal blood and cord blood by fluorescence quantitative RT‐PCR assay. According to the placental membranes pathological examination, pregnant women with PROM were divided into chorioamnionitis group (n=28) and non‐chorioamnionitis group (n=22). Results In the PROM group,the NF‐κB p65 and sTREM‐1 levels in maternal blood were significantly higher in women with chorioamnionitis than women without chorioamnionitis ( P <.05). The cutoff value of maternal NF‐κB p65, sTREM‐1, C‐reactive protein (CRP), and WBC level were 6.73, 2.93, 6.75 mg/L, and 10.8×10 9 /L, respectively, through analysis of the area under the ROC curve (AUC). The optimal combination test was detection of maternal blood NF‐κB p65 and CRP levels, which resulted in a sensitivity of 89.3% and a specificity of 72.7% for the prediction of subclinical chorioamnionitis. Conclusion Combined measurements of maternal NF‐κB p65 and CRP levels may be used as early biological indicators that predict subclinical chorioamnionitis in premature rupture of membranes.