Premium
The Immunogenicity of CRISP 1 Plasmid‐Based Contraceptive Vaccine can be Improved When Using Chitosan Nanoparticles as the Carrier
Author(s) -
Luo Jin,
Liu Xueli,
Zhang Yi,
Wang Yaqin,
Xu Wangming,
Yang Jing
Publication year - 2016
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12513
Subject(s) - dna vaccination , immunogenicity , lipofectamine , plasmid , zeta potential , chitosan , dna , transfection , microbiology and biotechnology , biology , chemistry , antigen , immunology , nanoparticle , recombinant dna , materials science , gene , biochemistry , vector (molecular biology) , nanotechnology
Problem To evaluate the effectiveness and security of a contraceptive vaccine using plasmid DNA encoding mouse CRISP 1 as antigen and chitosan nanoparticles as the carrier. Method of study Chitosan‐pc DNA 3.1‐ mCRISP 1 Nanoparticles ( CS ‐ DNA NP s) were prepared and characterized in terms of morphology, zeta potential, polydispersity index, and binding capacity of pDNA . The cytotoxicity and gene transfer capability of CS ‐ DNA NP s were assessed in COS ‐7 cells compared to Lipofectamine 2000 ™ . Four groups of mice received three injections of 0.9% normal saline, pc DNA 3.1 vector, pc DNA 3.1‐ CRISP 1, or CS ‐ DNA NP s, respectively. ELISA was used to examine the immune responses. Fertility and mean litter size were analyzed by natural mating. Results CS ‐ DNA NP s have a spherical or elliptical shape with a mean diameter of 189.3 nm, positive zeta potential, and good DNA condensation. It also showed high DNA se resistance and good transfection efficiency without cell toxicity. The titers of anti‐ mCRISP 1 antibodies from CS ‐ DNA NP ‐immunized mice were significantly higher than that of pc DNA 3.1‐ CRISP 1 group. Male and female CS ‐ DNA NP ‐immunized animals were recognized with a statistically significant reduction in their fertility compared with pc DNA 3.1‐ CRISP 1‐immunized mice. Conclusion This study demonstrated that using chitosan‐ DNA nanoparticles as the carrier can improve the immunogenicity of mCRISP 1 DNA contraceptive vaccine with good security.