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High Amounts of Total and Extracellular Vesicle‐Derived Soluble HLA‐G are Associated with HLA‐G 14‐bp Deletion Variant in Women with Embryo Implantation Failure
Author(s) -
Nardi Fabiola da Silva,
Slowik Renata,
Michelon Tatiana,
Manvailer Luis Felipe dos Santos,
Wagner Bettina,
Neumann Jorge,
Horn Peter,
Bicalho Maria da Graça,
Rebmann Vera
Publication year - 2016
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12507
Subject(s) - hla g , human leukocyte antigen , allele , extracellular vesicles , biology , embryo , andrology , extracellular , microbiology and biotechnology , gene , antigen , immunology , genetics , medicine
Problem Human leukocyte antigen‐G ( HLA ‐G) expression is related to 14‐bp insertion/deletion polymorphism at the 3′ UTR of the HLA ‐G gene. Soluble forms of HLA ‐G are released as free molecules or via extracellular vesicles ( EV s). Due to the crucial role of HLA ‐G during pregnancy, we analyzed the 14‐bp polymorphism and the two secreted forms in implantation failure women ( IF ) and in fertile women ( FW ). Method of study For the genetic analysis, 49 IF and 34 FW were genotyped. For sHLA ‐G quantification, serum samples from 35 IF and 23 FW were available. ExoQuick ™ kit was used for EV s precipitation. The total soluble HLA ‐G ( sHLA ‐G tot ) and vesicular sHLA ‐ G EV were quantified by ELISA . The EV s size and concentration were determined by nanoparticle tracking analysis (NTA). Results An increased proportion of IF presented high levels of sHLA ‐G tot ( P = 0.02) and vesicular sHLA ‐ G EV ( P = 0.0003) compared to FW . The 14‐bp deletion allele is more frequent in IF ( P = 0.0002) and associated with high levels of sHLA ‐G tot and vesicular sHLA ‐G EV . Conclusion The high expression of sHLA ‐G tot and sHLA ‐ G EV , together with the presence of the 14‐bp deletion allele, might be involved in implantation failure.