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Expression of Natural Cytotoxicity Receptors on and Intracellular Cytokine Production by NK Cells in Women with Gestational Diabetes Mellitus
Author(s) -
Chiba Hitomi,
Fukui Atsushi,
Fuchinoue Kohei,
Funamizu Ayano,
Tanaka Kanji,
Mizunuma Hideki
Publication year - 2016
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12491
Subject(s) - gestational diabetes , cytokine , flow cytometry , receptor , immunology , endocrinology , biology , medicine , andrology , pregnancy , gestation , genetics
Problem To determine the role of peripheral blood NK ( pNK ) cells in putative etiology of gestational diabetes, the expression of surface markers on p NK cells and the percentage of cytokine‐producing pNK cells in women at 12 weeks of pregnancy with gestational diabetes mellitus ( GDM ) were studied. Method of study Multicolor flow cytometry was used to analyze the expression of NK cell surface receptors ( CD 16, NK p46, and NK p30) and intracellular cytokines ( IFN ‐γ, TNF ‐α, TGF ‐β, and VEGF ) in pNK cells ( CD 56 dim and CD 56 bright ) at 12 weeks of pregnancy with GDM ( n = 7) and non‐ GDM ( n = 28). Results CD 56 bright / CD 16 − NK and CD 56 bright / NK p46 + NK cell percentage were significantly lower in GDM women than that in non‐ GDM women. IFN ‐γ‐ and TNF ‐α‐producing CD 56 + cells, respectively, were significantly high, while TGF ‐β‐ and VEGF ‐producing CD 56 + cells and CD 56 bright cells, respectively, were significantly low in GDM women. Conclusions Women with GDM possibly have abnormal NK cell function for the expression of surface receptors and cytokine production.