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Effect of Indoleamine 2,3‐Dioxygenase Expressed in HTR ‐8/ SV neo Cells on Decidual NK Cell Cytotoxicity
Author(s) -
Ban Yanli,
Zhao Ying,
Liu Fen,
Dong Baihua,
Kong Beihua,
Qu Xun
Publication year - 2016
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12481
Subject(s) - nkg2d , cytotoxicity , flow cytometry , biology , cell , cell culture , secretion , microbiology and biotechnology , in vitro , immunology , endocrinology , biochemistry , genetics
Problem To study the effect of indoleamine 2,3‐dioxygenase ( IDO ) expressed in HTR ‐8/ SV neo cells on NKG 2D and NK p46 expression and cytotoxicity of decidual NK ( dNK ) and peripheral NK ( pNK ) cells. Method of study CD 56 + dNK and pNK cells purified were cultured with HTR ‐8/ SV neo cell conditioned medium ( CM ), 1‐ MT + HTR ‐8/ SV neo cell CM , and complete RPMI 1640 medium (negative control) in vitro . The mRNA and protein expression of NKG 2D and NK p46 in NK cells were then assessed by qRT ‐ PCR and flow cytometry, respectively. Their cytotoxicity was evaluated with LDH assays, and TNF ‐α secretion was analyzed by ELISA. Results For dNK cells, the mRNA and protein expression of NK p46 as well as NKG 2D did not differ significantly among the three groups ( P > 0.05), whereas for pNK cells, the expression level was significantly decreased in HTR ‐8/ SV neo cell CM group than the other two groups ( P < 0.01). Peripheral NK cells cultured with HTR ‐8/ SV neo cell CM showed reduced cytotoxicity and TNF ‐α secretion than the other two groups ( P < 0.01), although there were no significant differences among three groups for dNK cells ( P > 0.05). Conclusion IDO expressed by HTR ‐8/ SV neo cells can down‐regulate NK p46 and NKG 2D expression and reduce cytotoxicity in pNK cells, and may contribute to keep dNK cytotoxicity at a low level, suggesting an important role for IDO in the maintenance of normal pregnancy.