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1,25‐Dihydroxy Vitamin D3 Modulates Endometriosis‐Related Features of Human Endometriotic Stromal Cells
Author(s) -
Delbandi AliAkbar,
Mahmoudi Mahmoud,
Shervin Adel,
Zarnani AmirHassan
Publication year - 2016
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12463
Subject(s) - stromal cell , endometriosis , angiogenesis , cytokine , vitamin , vitamin d and neurology , medicine , endocrinology , apoptosis , cancer research , andrology , biology , biochemistry
Problem We aimed to evaluate modulatory effects of vitamin D3 on endometriosis‐related features of endometriotic stromal cells. Method of study The effect of vitamin D3 on adhesion, invasion, proliferation, apoptosis, cytokine production, and angiogenesis potential of the eutopic (Eu ESC s), ectopic ( EESC s), and control ( CESC s) stromal cells from 25 women with and 20 women without endometriosis was investigated. Results In all groups, vitamin D3 significantly increased cell adhesion ( P = 0.0013–0.042), while decreased invasion ( P = 0.026–0.031) and proliferation ( P = 0.0013–0.039) of EESC s and Eu ESC s. Such treatment also resulted in a significant decrease in IL ‐6 production by EESC s ( P = 0.039), but had no significant effect on the IL ‐8 production. This vitamin also caused significant decrease in Bcl‐2 gene expression by Eu ESC s ( P = 0.04) and Bcl‐ xL by EESC s ( P = 0.044–0.009). In addition, vitamin D3 treatment reduced VEGF ‐A gene expression by EESC s ( P = 0.046–0.009). Conclusion Based on substantial favourable in vitro effects of vitamin D3 in endometriosis‐related features of human endometriotic stromal cells, further investigations on therapeutic potential of this hormone in endometriosis are warranted.