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IL ‐17 Induces Fetal Loss in a CBA /J× BALB /c Mouse Model, and an Anti‐ IL ‐17 Antibody Prevents Fetal Loss in a CBA /J × DBA /2 Mouse Model
Author(s) -
Xu WangMing,
Xiao ZhuoNi,
Wang XiaoBo,
Huang Ying
Publication year - 2016
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12437
Subject(s) - fetus , chemistry , antibody , andrology , immunology , microbiology and biotechnology , biology , medicine , pregnancy , genetics
Problem Many researchers have demonstrated that the expression of interleukin‐17( IL ‐17) is higher in spontaneous abortion. However, whether Th17 cells are an independent factor in inducing abortion is not known. Method of study This study investigated the effect of exogenous recombinant IL ‐17 and an anti‐ IL ‐17 antibody in a normal and an abortion mouse model using flow cytometry, enzyme‐linked immunosorbent assay, real‐time PCR , and Western blot. Results Th17 cells and the related factors, IL ‐17 and ROR γt, were significantly upregulated in abortion mice, and Treg cells and the related factor, Foxp3, were downregulated. Intraperitoneal injection of recombinant IL ( rIL )‐17 induced fetal loss in a normal mouse model, and an anti‐ IL ‐17 antibody prevented fetal loss in an abortion mouse model. Conclusion This study confirmed an imbalance of the Th17/Treg paradigm in abortion mice and IL ‐17 as a risk factor of fetal loss. An anti‐ IL ‐17 antibody may prevent abortion.