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Simultaneous Detection and Evaluation of Four Subsets of CD4+ T Lymphocyte in Lesions and Peripheral Blood in Endometriosis
Author(s) -
Takamura Masashi,
Koga Kaori,
Izumi Gentaro,
Hirata Tetsuya,
Harada Miyuki,
Hirota Yasushi,
Hiraike Osamu,
Fujii Tomoyuki,
Osuga Yutaka
Publication year - 2015
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12426
Subject(s) - endometriosis , lymphocyte , medicine , peripheral , endometrium , peripheral blood , flow cytometry , pathophysiology , t lymphocyte , lymphocyte subsets , pathology , peripheral blood lymphocyte , immunology , immune system , t cell
Problem The proportion of CD 4+ T lymphocytes, Th1, Th2, Th17, and regulatory T cells in endometriosis lesions and peripheral blood are not known. Method of study Lymphocytes were isolated from endometriosis lesions ( n = 10) and endometrium ( n = 10). Lymphocytes in peripheral blood were isolated from patients with and without endometriosis ( n = 10, 10). The CD 4+ T‐lymphocyte profile was analyzed by flow cytometry. Results The proportion of Th1 lymphocytes was significantly lower in endometriosis tissue (59.64 ± 9.2%) in comparison with endometrial tissue (79.07 ± 8.97%), whereas the Th17 lymphocyte fraction was significantly higher in endometriosis tissue (6.66 ± 2.53%) in comparison with endometrial tissue (2.27 ± 1.51%). Analysis of peripheral blood indicated that the Th1 proportion was significantly higher in women with endometriosis (10.25 ± 2.82%) in comparison with controls (6.96 ± 4.13%). Conclusion The CD 4+ T‐lymphocyte profile in lesions and peripheral blood is altered in women with endometriosis. These findings may help better understanding of T‐lymphocyte involvement in the pathophysiology of endometriosis.