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The Receptor for the CD 200 Tolerance‐Signaling Molecule Associated with Successful Pregnancy is Expressed by Early‐Stage Breast Cancer Cells in 80% of Patients and by Term Placental Trophoblasts
Author(s) -
Clark David A.,
DhesyThind Sukhbinder,
Arredondo Jorge L.,
Ellis Peter M.,
Ramsay Jennifer A.
Publication year - 2015
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12403
Subject(s) - trophoblast , receptor , biology , cancer cell , microbiology and biotechnology , cancer research , syncytiotrophoblast , cancer , placenta , immunology , fetus , pregnancy , biochemistry , genetics
Problem The CD 200 tolerance‐signaling molecule that is expressed by a wide variety of tissues, including placental trophoblast and epithelial tumor cells, lacks an intracytoplasmic tail and must act by binding to CD 200 receptors that have a limited expression on lymphomyeloid cells. This binding can inhibit inflammation and NK cells, promote macrophage secretion of indoleamine‐2,3 dioxygenase ( IDO ), and promote generation of Treg cells. Recently, CD 200R1 was reported on human first trimester placental villous trophoblast cells. CD 200R1 has not been described on malignant tumor cells. As malignant tumor cells exhibit a number of characteristics of trophoblast, is CD 200R1 expressed? Method of study Affinity‐purified rabbit polyclonal antibodies to CD 200 and CD 200R1 were used to immunostain tissue blocks available from cases in a previous cross‐sectional study of Stage 1‐ IIIA human breast cancer cases and term placental trophoblast. Results Affinity‐purified anti‐ CD 200R1 stained primary breast cancer cells and term placental villous trophoblasts. Tumor cells were also stained by anti‐ CD 200 as in a previous study (correlation P = 0.0042), but CD 200R1 and CD 200 were not correlated. Presence or absence of strong CD 200 expression in the tumor did not correlate with metastasis, and a similar result was obtained with CD 200R1. Conclusions This is the first report of CD 200R1 expression by human epithelial tumor cells, and specifically, early‐stage human breast cancer cells. It is also the first report of CD 200R1 expression by term placental villous trophoblasts. The potential biological significance of CD 200R1 expression in non‐hematopoietic cells is discussed.