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Determination of CD64 for the Diagnosis of Bacterial Chronic Prostatitis
Author(s) -
Qian Li,
Li ShiBao,
Zhou Yan,
Teng ShiJie,
Guo JingJing
Publication year - 2015
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12391
Subject(s) - flow cytometry , cd64 , prostatitis , monoclonal antibody , antibody , immunology , biology , pathology , microbiology and biotechnology , medicine , prostate , cancer
Problem CD 64 is a 72‐ kD a transmembrane glycoprotein, the highest affinity receptor of immunoglobulin (IgG), and a member of the IgG superfamily. The CD 64 expression by leukocytes in the prostatic fluid was determined by flow cytometry to explore the clinical significance of this glycoprotein in the diagnosis of chronic prostatitis (CP). Method of study Prostatic fluid samples collected from 116 CP patients and 27 normal cases were subjected to bacterial culture and leukocyte CD 64 expression determination using flow cytometry with monoclonal antibodies. Results The leukocyte CD 64 expression levels of the pathogen groups were significantly higher than those of the normal groups, especially those of Staphylococcus simulans (3879.26 ± 764.52/cell) and Staphylococcus auricularis (2903.14 ± 137.61/cell). The leukocyte CD 64 expression in the prostatic fluid was more rapidly and accurately detected by flow cytometry than by bacterial culture. Conclusions CD 64 expression by leukocytes in the prostatic fluid correlated with bacterial infection and may serve as a marker for the early diagnosis of CP.