Premium
A New Enzyme‐linked Sorbent Assay ( ELSA ) to Quantify Syncytiotrophoblast Extracellular Vesicles in Biological Fluids
Author(s) -
Göhner Claudia,
Weber Maja,
Tannetta Dionne S.,
Groten Tanja,
Plösch Torsten,
Faas Marijke M.,
Scherjon Sicco A.,
Schleußner Ekkehard,
Markert Udo R.,
Fitzgerald Justine S.
Publication year - 2015
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12367
Subject(s) - syncytiotrophoblast , nanoparticle tracking analysis , extracellular vesicles , ex vivo , chemistry , placenta , annexin , alkaline phosphatase , microvesicles , extracellular , chromatography , enzyme , biochemistry , biology , microbiology and biotechnology , fetus , in vitro , pregnancy , microrna , genetics , gene
Problem The pregnancy‐associated disease preeclampsia is related to the release of syncytiotrophoblast extracellular vesicles ( STBEV ) by the placenta. To improve functional research on STBEV , reliable and specific methods are needed to quantify them. However, only a few quantification methods are available and accepted, though imperfect. For this purpose, we aimed to provide an enzyme‐linked sorbent assay ( ELSA ) to quantify STBEV in fluid samples based on their microvesicle characteristics and placental origin. Method of Study Ex vivo placenta perfusion provided standards and samples for the STBEV quantification. STBEV were captured by binding of extracellular phosphatidylserine to immobilized annexin V. The membranous human placental alkaline phosphatase on the STBEV surface catalyzed a colorimetric detection reaction. Results and Conclusion The described ELSA is a rapid and simple method to quantify STBEV in diverse liquid samples, such as blood or perfusion suspension. The reliability of the ELSA was proven by comparison with nanoparticle tracking analysis.