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Maternal Lipopolysaccharide Exposure Promotes Immunological Functional Changes in Adult Offspring CD 4 + T Cells
Author(s) -
Luan Rong,
Cheng Hao,
Li Lin,
Zhao Qiang,
Liu Hui,
Wu Zhenzhou,
Zhao Liqing,
Yang Jinghua,
Hao Jianlei,
Yin Zhinan
Publication year - 2015
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12364
Subject(s) - offspring , immune system , spleen , biology , lipopolysaccharide , endocrinology , medicine , tumor necrosis factor alpha , immunology , andrology , pregnancy , genetics
Problem Maternal immune activation ( MIA ) is a risk factor for autism and schizophrenia. However, how MIA affects offspring immune function remains unknown. Method of study To investigate the effect of MIA on the offspring, pregnant C57 BL /6J mice were given an intraperitoneal injection of 50 μg/kg lipopolysaccharide ( LPS ) on gestational day 12.5. Results Adult LPS ‐treated offspring were hyper‐reactive to LPS , and enhanced tumor necrosis factor‐α production was observed. CD 4 + T cells from LPS offspring had an elevated percentage of interferon ( IFN )‐γ + CD 4 + T cells and interleukin ( IL )‐17A + CD 4 + T cells in the spleen, IL ‐17A + CD 4 + T cells in the liver, and CD 4 + Foxp3 + T cells in the spleen. LPS offspring CD 4 + T cells showed increased proliferation and an enhanced survival rate. DNA microarray analysis of resting LPS offspring CD 4 + T cells identified eight up‐regulated genes, most of which encoded transcription factors. Quantitative liquid chromatography–mass spectrometry identified 18 up‐regulated proteins in resting LPS offspring CD 4 + T cells and five up‐regulated proteins in activated LPS offspring CD 4 + T cells, most of which participated in the PANTHER Gene Ontology metabolic process. Conclusions Our results showed that MIA to LPS up‐regulated proteins involved in metabolic process in CD 4 + T cells from LPS offspring that might contribute to the hyperactivated immune response of adult LPS offspring.