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Cyclic Stretch Augments Production of Neutrophil Chemokines, Matrix Metalloproteinases, and Activin A in Human Endometrial Stromal Cells
Author(s) -
Izumi Gentaro,
Koga Kaori,
Nagai Miwako,
Urata Yoko,
Takamura Masashi,
Harada Miyuki,
Hirata Tetsuya,
Hirota Yasushi,
Ogawa Kenji,
Inoue Satoshi,
Fujii Tomoyuki,
Osuga Yutaka
Publication year - 2015
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12359
Subject(s) - chemotaxis , cxcl1 , stromal cell , endometrium , matrix metalloproteinase , chemokine , decidualization , endocrinology , interleukin 8 , medicine , secretion , uterus , chemistry , microbiology and biotechnology , biology , inflammation , receptor
Problem The aim of this study was to test our hypothesis: Contractile activity that occurs in the uterus during menstruation induces biochemical factors that enhance remodeling of the endometrium. Method of Study Cyclic stretch, mimicking contractile activity during menstruation, was applied to human endometrial stromal cells ( ESC ) using the Flexercell Tension system. The concentration and activity of CXCL 8, CXCL 1, MMP s, and activin A were measured using ELISA s and specific assays. Neutrophil chemotactic activity was evaluated using migration assays. Results Cyclic stretch significantly induced ESC secretion of CXCL 8 and CXCL 1 and neutrophil chemotaxis. Stretch also increased MMP ‐1, MMP ‐2, and MMP ‐3 activity, activin A secretion, and activity in ESC . Conclusion These results indicate that the contractile activities of the uterus during menstruation contribute to the remodeling of the endometrium, by inducing chemokine secretion, MMP expression, activity, and neutrophil chemotaxis.