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Fn14 Receptor Appears as a Modulator Of Ovarian Steroid‐Related Regulation Of Goat Endometrial Epithelial Cell IL ‐18 expression
Author(s) -
Lei Mingzhu,
Qin Lijuan,
Wang Aihua,
Jin Yaping,
Zhao Xianjun,
Qi Xuefeng
Publication year - 2015
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12343
Subject(s) - gene knockdown , stromal cell , endometrium , apoptosis , receptor , biology , endocrinology , steroid , medicine , cancer research , chemistry , microbiology and biotechnology , hormone , biochemistry
Problem Tumor necrosis factor‐like weak inducer of apoptosis ( TWEAK )/fibroblast growth factor‐inducible 14 (Fn14) interactions affect the regulation of cytotoxic/immunotrophic pathways that are themselves under control of IL ‐18. The effect of Fn14 on regulation of endometrium IL ‐18 expression, however, remains unclear. Method and study The aim was to determine the mode of ovarian steroid action in regulating Fn14 expression by goat endometrial epithelial cells ( EEC s) in the presence and absence of endometrial stromal cells ( ESC s). The possible role of Fn14 on the expression of IL ‐18 by EEC s was also evaluated. Results Opposite effects of E 2 and/or P 4 on the regulation of both Fn14 mRNA and protein expression by EECs were observed in the presence and absence of ESCs. Fn14 knockdown by blocking antibody or siRNA resulted in a decrease of IL‐18 mRNA and protein levels in EECs cocultured with ESCs, and no significant difference of the IL‐18 mRNA and protein levels in the EECs was observed between steroid treatment group and control group. Conclusion These findings confirm the importance of steroids in controlling Fn14 expression in goat EEC s. Furthermore, Fn14 appears as a novel modulator of the steroid‐related IL ‐18 expression in EEC s in the presence of ESC s.