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Purified IgG from Patients with Obstetric but not IgG from Non‐obstetric Antiphospholipid Syndrome Inhibit Trophoblast Invasion
Author(s) -
Poulton Katie,
Ripoll Vera M.,
Pericleous Charis,
Meroni Pier Luigi,
Gerosa Maria,
Ioannou Yiannis,
Rahman Anisur,
Giles Ian P.
Publication year - 2015
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12341
Subject(s) - trophoblast , immunology , antiphospholipid syndrome , immunoglobulin g , tlr4 , andrology , cytokine , messenger rna , p38 mitogen activated protein kinases , phosphorylation , antibody , medicine , biology , pregnancy , mapk/erk pathway , immune system , placenta , microbiology and biotechnology , fetus , gene , biochemistry , genetics
Problem Some patients with antiphospholipid syndrome (APS) suffer pregnancy morbidity (PM) but not vascular thrombosis (VT), whilst others suffer VT only. Therefore, we compared the effects of IgG from VT+/PM− and VT−/PM+ subjects on human first‐trimester trophoblast (HTR8) cells. Method of study HTR‐8 cells were incubated with APS VT+/PM−, APS VT−/PM+ or healthy control (HC) IgG. We measured trophoblast invasion by cell invasion assay; mRNA expression of TLR4 and adaptor proteins; phosphorylation of p38 MAPK, NFκB and ERK; and expression of interleukin (IL)‐8 and IL‐6. Results VT−/PM+ IgG, but not VT+/PM− IgG significantly reduced HTR‐8 invasion. The effects on invasion were blocked by TLR‐4 inhibition. Neither VT+/PM− nor VT−/PM+ IgG altered MyD88 mRNA expression, phosphorylation of signalling molecules or cytokine expression. Conclusions VT−/PM+ IgG exert functionally relevant effects on human trophoblast cells but VT+/PM− IgG do not.