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Transcriptomic Profiling of Human Choriodecidua During Term Labor: Inflammation as a Key Driver of Labor
Author(s) -
Stephen Gillian L.,
Lui Sylvia,
Hamilton Sarah A.,
Tower Clare L.,
Harris Lynda K.,
Stevens Adam,
Jones Rebecca L.
Publication year - 2015
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12328
Subject(s) - decidua , myometrium , downregulation and upregulation , transcriptome , inflammation , rna , biology , andrology , preterm labor , cervix , microbiology and biotechnology , gene expression profiling , gene , gene expression , placenta , endocrinology , uterus , immunology , medicine , pregnancy , genetics , fetus , cancer
Problem Inflammation is a driver of labor in myometrium and cervix; however, the involvement of decidua is poorly defined. We have reported decidual leukocyte infiltration prior to and during labor; the regulators of these inflammatory processes are unknown. Method of study Choriodecidua RNA obtained after term labor or elective cesarean delivery was applied to Affymetrix GeneChips. Pathway analysis and gene validation were performed. Results Extensive inflammatory activation was identified in choriodecidua following labor, predominantly upregulation of genes regulating leukocyte trafficking and cytokine signalling. Genes governing cell fate, tissue remodelling, and translation were also altered. Upregulation of candidate genes ( ICAM 1 , CXCR 4 , CD 44 , TLR 4 , SOCS 3 , BCL 2A, and IDO ) was confirmed. NF κB, STAT 1&3, HMGB 1, and mi RNA ‐21, mi RNA ‐46, mi RNA ‐141, and mi RNA ‐200 were predicted upstream regulators. Conclusion This study confirms inflammatory processes are major players in labor events in choriodecidua, as in other gestational tissues. Suppressing uterine inflammation is likely to be critical for arresting premature labor.