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CD 4 + F oxp3 + R egulatory T Cells in Autoimmune O rchitis: Phenotypic and Functional Characterization
Author(s) -
Jacobo Patricia,
Guazzone Vanesa A.,
Pérez Cecilia V.,
Lustig Livia
Publication year - 2015
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12312
Subject(s) - flow cytometry , phenotype , foxp3 , orchitis , microbiology and biotechnology , biology , endocrinology , immunology , medicine , chemistry , pathology , immune system , gene , biochemistry
Problem The phenotype and function of regulatory T ( T reg) cells in rats with experimental autoimmune orchitis ( EAO ) was evaluated. Method of study Distribution of T reg cells in draining lymph nodes from the testis ( TLN ) and from the site of immunization ( ILN ) was analysed by immunohistochemistry. The number, phenotype and proliferative response (5‐bromo‐2′‐deoxyuridine incorporation) of T reg cells were evaluated by flow cytometry and T reg cell suppressive activity by in vitro experiments. TGF ‐β expression was evaluated by immunofluorescence. Results Absolute numbers of T reg cells and B rd U + T reg cells were increased in LN from experimental compared to normal and control rats. These cells displayed a CD 45 RC − , CD 62L − , Helios + phenotype. CD 4 + CD 25 bright T cells from TLN of experimental rats were able to suppress T cell‐proliferation more efficiently than those derived from normal and control rats. Cells isolated from TLN and ILN expressed TGF ‐β. Conclusion Our results suggest that T reg cells with a memory/activated phenotype proliferate extensively in the inflamed testis and LN of rats with EAO exhibiting an enhanced suppressive capacity. TGF ‐β may be involved in their suppressive mechanism.