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Secondary Biliary Cholestasis Promotes Testicular Macrophage Infiltration and Autophagy in Rats
Author(s) -
Shi ShaoHua,
Jiang Li,
Xie HaiYang,
Zhu YouFa,
Zhang WenJin,
Zheng ShuSen
Publication year - 2015
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12292
Subject(s) - cholestasis , autophagy , medicine , inflammation , infiltration (hvac) , endocrinology , decompression , biliary tract , vacuole , bile duct , necrosis , mitophagy , steatosis , biology , surgery , biochemistry , apoptosis , cytoplasm , physics , thermodynamics
Problem Cholestasis can cause translocation of gut bacteria, and endotoxemia, and systemic inflammation. Now, little is known about the effects of cholestasis on the testicular inflammation and autophagy. Methods A rat biliary cholestasis model caused by common bile duct ligation ( CBDL ), together with biliary decompression (choledochoduodenostomy), was used. Results The magnitude of MCP ‐1 expression and CD 68 + macrophage infiltration within testes was progressively up‐regulated in rats along with increasing duration of CBDL and was maintained at relatively high level in rats with biliary decompression. The large up‐regulation of testicular ATG ‐12, LC 3II, and autophagic vacuoles was found with the extending duration of CBDL and kept at 5 weeks following biliary decompression. The autophagic contents were a large accumulation of mitophagy in testes in rats with CBDL , and cytosol components in rats with biliary decompression. Conclusion Secondary biliary cholestasis can promote inflammatory reaction and the activation of mitophagy and autophagy in testes.