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Novel Morphological Findings of Human Sperm Removal by Leukocytes in In Vivo and In Vitro Conditions: Preliminary Study
Author(s) -
Piasecka Malgorzata,
Fraczek Monika,
Gaczarzewicz Dariusz,
Gill Kamil,
SzumalaKakol Anna,
Kazienko Anna,
Laszczynska Maria,
Lenart Stanislaw,
Beutin Lothar,
Kurpisz Maciej
Publication year - 2014
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12284
Subject(s) - in vivo , phagocytosis , sperm , in vitro , biology , andrology , semen , reproductive immunology , immune system , microbiology and biotechnology , immunology , anatomy , reproductive biology , embryo , embryogenesis , biochemistry , medicine
Problem Biological significance of increased number of leukocytes in ejaculate remains a subject of controversy. The aim of this research was to describe the morphological characteristics of the sperm elimination by leukocytes in in vivo and in vitro conditions using natural stimulator of the immune system—uropathogenic E scherichia coli , O75: HNT , isolated from semen. Method of study The study was performed on ejaculated spermatozoa from normozoospermic men with leukocytospermia ( n = 10, in vivo experiment) or without leukocytospermia ( n = 15, in vitro experiment). Morphological observations were performed using light and scanning electron microscopy. Results Sperm removal by active leukocytes mediated by traditional phagocytosis and generation of extracellular traps were observed in in vivo and in vitro experiments. Conclusion Our morphological data suggest that human germ cells activate leukocytes triggering both traditional phagocytosis and a novel trapping mechanism, followed by extensive sperm elimination.