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Chlamydia muridarum Infection Associated Host Micro RNA s in the Murine Genital Tract and Contribution to Generation of Host Immune Response
Author(s) -
Gupta Rishein,
Arkatkar Tanvi,
Yu JiehJuen,
Wali Shradha,
Haskins William E.,
Chambers James P.,
Murthy Ashlesh K.,
Bakar Sazaly Abu,
Guentzel M Neal,
Arulanandam Bernard P.
Publication year - 2015
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12281
Subject(s) - chlamydia trachomatis , chlamydia , biology , immune system , pathogenesis , immunology , rna , sex organ , heat shock protein , host (biology) , microrna , microbiology and biotechnology , virology , gene , genetics
Problem Chlamydia trachomatis ( CT ) is the leading sexually transmitted bacterial infection in humans and is associated with reproductive tract damage. However, little is known about the involvement and regulation of micro RNA s (miRs) in genital CT . Methods We analyzed miRs in the genital tract ( GT ) following C. muridarum (murine strain of CT ) challenge of wild type ( WT ) and CD 4 + T‐cell deficient ( CD 4 −/− ) C57 BL /6 mice at days 6 and 12 post‐challenge. Results At day 6, miRs significantly downregulated in the lower GT were miR‐125b‐5p, ‐16, ‐214, ‐23b, ‐135a, ‐182, ‐183, ‐30c, and ‐30e while ‐146 and ‐451 were significantly upregulated, profiles not exhibited at day 12 post‐bacterial challenge. Significant differences in miR‐125b‐5p (+5.06‐fold change), ‐135a (+4.9), ‐183 (+7.9), and ‐182 (+3.2) were observed in C. muridarum ‐infected CD 4 −/− compared to WT mice. In silico prediction and mass spectrometry revealed regulation of miR‐135a and ‐182 and associated proteins, that is , heat‐shock protein B1 and alpha‐2 HS ‐glycoprotein. Conclusion This study provides evidence on regulation of miRs following genital chlamydial infection suggesting a role in pathogenesis and host immunity.